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high-resolution melting analysis (hrma)  (PrimerDesign Inc)

 
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    PrimerDesign Inc high-resolution melting analysis (hrma)
    High Resolution Melting Analysis (Hrma), supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high-resolution melting analysis (hrma)/product/PrimerDesign Inc
    Average 90 stars, based on 1 article reviews
    high-resolution melting analysis (hrma) - by Bioz Stars, 2026-04
    90/100 stars

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    Image Search Results


    Journal: bioRxiv

    Article Title: Functional Landscape of Zebrafish Gonadotropins and Receptors: A Comprehensive Genetic Analysis

    doi: 10.1101/2025.11.26.690707

    Figure Lengend Snippet:

    Article Snippet: The extracted genomic DNA was analyzed using High-Resolution Melt Analysis (HRMA) with the Precision Melt Analysis software (Bio-Rad Laboratories, Hercules, CA) to identify different genotypes based on melt curve differences [ ].

    Techniques:

    Development of a Zebrafish CRISPR/Cas9 pipeline to assess functional impact of DMD modifier candidates . (A) Adult fish heterozygous for dmd are intercrossed to generate embryos for the screen. Because DMD in zebrafish follows an autosomal recessive inheritance pattern, one-quarter of the intercross progeny are homozygous dmd mutants ( dmd-/- ), all of which are identifiable using birefringence imaging by 4 dpf due to the presence of muscle lesions. Homozygous wild-type ( dmd+/+ ) and heterozygous dmd ( dmd+/- ) fish are indistinguishable. Embryos are injected by the 1-cell stage with modifier-targeting CRISPR and Cas9 protein or raised as uninjected controls. All fish are assayed for DMD onset between 2-4 dpf using live birefringence imaging. At 4 dpf, all fish are fixed, birefringence imaged, and genotyped via HRMA. (B, B’) Representative examples of wild-type (WT, top) and dmd-/- mutant (bottom) fish imaged via birefringence at 4 dpf. The boxes in B are shown magnified in B’. Wild-type fish show strong, uniform birefringence, whereas dmd mutant fish have variably-sized non-birefringence patches indicating dystrophic lesions (white arrows). Fish containing one or more lesion, regardless of lesion size or number, are scored as dystrophic in the onset assay.

    Journal: bioRxiv

    Article Title: Validation of Duchenne muscular dystrophy candidate modifiers using a CRISPR-Cas9-based approach in zebrafish

    doi: 10.1101/2025.05.20.655139

    Figure Lengend Snippet: Development of a Zebrafish CRISPR/Cas9 pipeline to assess functional impact of DMD modifier candidates . (A) Adult fish heterozygous for dmd are intercrossed to generate embryos for the screen. Because DMD in zebrafish follows an autosomal recessive inheritance pattern, one-quarter of the intercross progeny are homozygous dmd mutants ( dmd-/- ), all of which are identifiable using birefringence imaging by 4 dpf due to the presence of muscle lesions. Homozygous wild-type ( dmd+/+ ) and heterozygous dmd ( dmd+/- ) fish are indistinguishable. Embryos are injected by the 1-cell stage with modifier-targeting CRISPR and Cas9 protein or raised as uninjected controls. All fish are assayed for DMD onset between 2-4 dpf using live birefringence imaging. At 4 dpf, all fish are fixed, birefringence imaged, and genotyped via HRMA. (B, B’) Representative examples of wild-type (WT, top) and dmd-/- mutant (bottom) fish imaged via birefringence at 4 dpf. The boxes in B are shown magnified in B’. Wild-type fish show strong, uniform birefringence, whereas dmd mutant fish have variably-sized non-birefringence patches indicating dystrophic lesions (white arrows). Fish containing one or more lesion, regardless of lesion size or number, are scored as dystrophic in the onset assay.

    Article Snippet: DNA was extracted from adult fin tissue or whole larvae as described above and 1ul was used as template in a 10ul HRMA reaction (BioRad 172-5112) in a CFX Duet Real-Time PCR System (#12016265) and analyzed using BioRad Precision Melt Analysis Software.

    Techniques: CRISPR, Functional Assay, Imaging, Injection, Mutagenesis